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Optimizing a Corning® Epic® Biochemical Assay: An Example with the Lactate Dehydrogenase A
09 Aug 2010: This application note show that the Corning® Epic® System enables label-free direct biochemical assay for small molecule binding to an immobilized protein. Several simple steps can be performed and are necessary to optimize the assay, including selecting the right pH and buffer conditions, optimizing concentration of target bound, and evaluating assay robustness with respect to buffer conditions.
Corning Life Sciences
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Corning® Epic® Cell-based Assays: Tolerance to DMSO Mismatching in Antagonist Assays
09 Aug 2010: This application note shows that to maximize the sensitivity of an Epic® assay, a buffer should match as closely as possible. Quantities of DMSO added greater than a mismatch of 1% result in a loss of assay sensitivity, however the IC50 values are not greatly affected.
Corning Life Sciences
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Adhesive Micropatterns for HCS: a Case Study with Blebbistatin
29 Jun 2010: This poster demonstrates that drug effects can be detected and measured in the submicromolar range, when they are barely observable on regular cell culture supports. In addition, a significant difference between treated and untreated cells is detected with only 20 analyzed cells.
CYTOO Inc.
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Expansion and Differentiation of H7 Human Embryonic Stem Cells in Chemically Defined Medium Using Corning® Synthemax™ Surface
21 Jun 2010: In this application note Corning Synthemax Surface is used to support long-term self-renewal and differentiation of H7 hESCs. Cells demonstrated a stable doubling time, high level of expression of hESC phenotypic markers (Oct4, SSEA4 and TRA-1-60) and normal karyotype. Corning Synthemax Surface also supported successful directed differentiation of H7 cells into functional cardiomyocytes, proving suitability of Corning Synthemax Surface for both expansion and differentiation of hESC culture.
Corning Life Sciences
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Human Oncostatin M is Functionally Equivalent to Mouse LIF in Supporting Mouse ES Cell Pluripotency & Germline Competency
21 Jun 2010: In this application note R&D Systems demonstrate human OSM is able to maintain ES cell pluripotency in a similar manner to mouse LIF, including long-term maintenance of pluripotency and the ability to differentiate in vitro, the ability to activate STAT3, and germline transmission competency. The study suggests that OSM may be an additional factor that plays a role in early embryonic development and that it may be used as an alternative to LIF in mouse ES cell culture.
R&D Systems, Inc.
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Microplate-Based Endotoxin Testing in GLP/GMP Environments with the VersaMax™ Absorbance Microplate Reader and Pyrochrome® Assay
07 Jun 2010: This application note describes a typical set-up for a standard curve in the Pyrochrome® Assay. The Pyrochrome® Assay together with the VersaMax Microplate Reader offers a sensitive and specific method for detecting endotoxin contamination in the production process of the pharmaceutical and medical device industries.
Molecular Devices
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LpxC RapidFire® Screening Analysis
03 Jun 2010: LpxC represents an attractive target for therapeutics. This application note presents a RapidFire high‐throughput mass spec method for both the gram positive and the gram negative bacteria LpxC enzyme. This method screens the LpxC catalyzed reaction using the native reaction substrate, resulting in significant number of hits for client lead discovery programs.
BIOCIUS Life Sciences
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High-Throughput Low-Volume DNA Quantification and Analysis
03 Jun 2010: This application note details the very high speed at which the Thermo Scientific Multiskan GO can perform full DNA quantification and spectral analysis on large numbers of low volume (5 - 10 µl) samples. With the Multiskan® GO, measurement of full DNA spectra between 220-380 nm from 96 samples can be performed in less than three minutes, providing a spectral measuring capacity of over 2,000 samples per hour.
Thermo Fisher Scientific
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Low pI Protein Immobilization Strategy: Cationization
24 May 2010: This application note describes how Epic biochemical assays were used to investigate and Immobilize two different low pI proteins: trypsin inhibitor (from Glycine max, 21.5 kDa, pI 4.6) and pepsin A (hog stomach, 41.3 kDa,pI 4.0).
Corning Life Sciences
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